Evidence for genetic distinction among sympatric ecotypes of Arctic char (Salvelinus alpinus) in south‐western Alaskan lakes

Resource polymorphism may play an important role in the process of speciation. The Arctic char (Salvelinus alpinus) exhibits great phenotypic and genetic diversity across its range, making it an ideal species for studies of resource polymorphism and divergence. Here, we investigated genetic variation at 11 microsatellite loci among 287 Arctic char from five isolated yet proximate postglacial lakes in south‐western Alaska that were previously examined for resource polymorphism. Significant differences in pairwise F_ST were detected among all lakes (range from 0.05 to 0.28, all P < 0.02). In one lake (Lower Tazimina Lake), we found evidence for two genetic groups of char and for significant differences in the distribution of microsatellite variability among at least two of the three previously described body size morphotypes (‘large’‐, ‘medium’‐, and ‘small’‐bodied char; maximum F_ST = 0.09; differences in admixture proportions). We also found a significant association between genetic admixture proportions and gill raker counts among body size morphs (r = ?0.73, P < 0.001). Our data represent the first record of genetically distinct sympatric morphs of Arctic char in Alaska and provide further evidence that differences in morphology associated with feeding (gill rakers) and growth trajectories reflect niche diversification and promote genetic divergence in Holarctic populations of Arctic char.     

Relative Prevalence of Antibiotic Resistance among Heterotrophic Bacteria from Natural and Culture Environments of Freshwater Prawn,Macrobrachium Rosenbergii

As antibiotic residues and multidrug‐resistant (MDR) bacteria are of increasing concern to those involved in the seafood industry, there is an intense international focus to monitor MDR bacteria in seafood. There is also a trend to source seafood from organically raised farms in order to reduce antibiotic usage in culture operations. Hence this study was conducted to compare the antibiotic resistance among bacteria associated with various samples from the natural and culture environment of Macrobrachium rosenbergii. Samples from the natural environment included water, sediment, eggs, larvae, postlarvae (PL), feed items, and gut samples of M. rosenbergii and those from the culture environment were water, sediment, eggs, feed pellets, and gut samples of M. rosenbergii. Bacteria from water samples of natural and culture environments were frequently resistant to ampicillin, erythromycin, and penicillin, while more than 50% of the strains from sediment were resistant to erythromycin, nalidixic acid, and penicillin. Bacterial isolates from commercial feed had a relatively high degree of antibiotic resistance. Interestingly, occurrence of multiple drug resistance was higher among the bacteria associated with the samples from the natural environment of M. rosenbergii, pointing to more favorable selection pressure for drug resistant mutants in the natural environment.     

Tape depth and germination method influence patterns of salt accumulation with subsurface drip irrigation

Subsurface drip irrigation (SDI) can result in accumulation of soluble salts at or near the soil surface. In the southwestern USA, rainfall is usually inadequate for stand establishment, thus supplemental irrigation is necessary. Use of sprinklers to minimize salt concentrations near the soil surface is an alternative to using SDI for stand establishment. Our objective was to evaluate the effects of germination method (irrigation with SDI or sprinklers), depth of SDI tape (0.18 and 0.25 m), and irrigation water salinity (1.5 and 2.6 dS m⁻¹) on salt and Br distribution after each of two consecutive growing seasons. Treatments consisted of factorial combinations of these three factors. Bromide was used to trace salt accumulation from the drip tape. After season 1, the highest salt concentrations (ECe up to 11 dS m⁻¹) were in the top 3 cm of soil. Below 3 cm, soil EC dropped significantly and remained constant to 1.05 m. Similarly, Br concentrations were highest in the top 3 cm of soil. The mass of salt and Br recovered in the top 3 cm were significantly affected by tape depth, and water EC significantly affected salt mass. Salt present in the soil after season 1 adversely affected crop emergence in season 2, where SDI was used for stand establishment. After season 2, the highest salt and Br concentrations were at about 25 cm depth, probably due to 210 mm of rainfall that occurred near the end of the growing season. There were no significant differences among treatments in the mass of either salt or Br in the top 3 cm or 16 cm of the soil profile after season 2. Timely rainfall, transplanting rather than direct seeding, and changing bed geometry can reduce dependence on sprinklers for stand establishment.     

Water use estimates for various rice production systems in Mississippi and Arkansas

Rice irrigation-water use was estimated in Mississippi (MS) and Arkansas (AR) in 2003 and 2004. Irrigation inputs were compared on naturally sloping (i.e. contour-levee system) and mechanically graded fields. In MS, rice production consumed, on average, 895 mm water, but irrigation inputs were greatly affected by production system. Contour-levee systems accounted for 35% of the production area and consumed 1,034 mm irrigation. Fields mechanically graded to a consistent slope of approximately 0.1% (i.e. straight-levee systems) consumed 856 mm irrigation and accounted for 60% of the production area. Fields devoid of slope (i.e. zero-grade system) accounted for 5% of the production area and consumed 382 mm irrigation. In AR, contour-levee rice production consumed 789 mm compared to 653 mm with a straight-levee system. Using low pressure, thin wall (9–10 mil) disposable irrigation tubing to deliver water to each paddy independently reduced irrigation inputs by 28% in MS and 11% in AR when compared to a single-point (levee-gate) distribution system.     

A non-linear model to predict crown recession of Norway spruce (<Emphasis Type="Italic">Picea abies</Emphasis> [L.] Karst.) in Austria

In this study, a non-linear model was developed that predicts the five-year change of height to crown base (HCB) of Norway spruce (Picea abies [L.] Karst.). Data were available from the Austrian National Forest Inventory and comprised 2,419 trees from 1,637 permanent sample plots measured during 1981 and 2002. The dynamic model explained 36% of the variation in the observed change of HCB. It is well behaved and meets biological expectations. Based on five independent data sets, the predictive ability of the new dynamic model was compared to an already existing static crown ratio model. For this comparison, the models were applied as follows: the new ∆ HCB model was used to predict the change in HCB directly. For the semi-dynamic method, the static model was applied at the end and at the start of the growth period to obtain two estimates of HCB. The difference of these two estimates was then added to the initial HCB. For the static method, the model was only applied at the end of the prediction period to obtain the new estimate of HCB. Except for one plot, the new ∆ HCB model yielded the smallest BIAS and the highest precision, followed by the semi-dynamic and the static method. Because the independent data sets cover a broad range of age classes and thinning regimes, the validation results also indicate that the new ∆ HCB is robust and the effect of stand management is adequately represented.     

Plant Location Patterns in the European Automobile Supplier Industry

Motor vehicle and motor vehicle parts production tend to exhibit a strong degree of agglomeration. We estimate a location model utilizing detailed plant‐level data for over 1,700 European locations of the largest motor vehicle parts suppliers, as well as the location of all light vehicle assembly plants operational in 2010. This unique data set allows us to estimate a location model for the region at a significantly higher level of resolution than the previous literature. The results suggest that the main forces of agglomeration in the European auto supplier sector are highway access and the desire to locate near assembly plants as well as near other parts‐producing plants.     

高压静电喷雾纯天然有机制剂"泰栢一号"对蔬菜蚜虫的防治效果

"泰栢一号"是一种纯天然有机制剂,本文以吡虫啉为对照药剂,分别在室内与田间测试了在常规使用和在高压静电喷雾条件下"泰栢一号"对蔬菜蚜虫的防治效果,结果表明,"泰栢一号"40X稀释液处理蚜虫48h后死亡率为3.36%,清水对照处理的死亡率为3.33%,二者间无显著性差异,而吡虫啉10 mg/L处理24h蚜虫的死亡率为78.86%."泰栢一号"与高压静电喷雾器配合使用则表现出良好的杀虫效果,200×稀释液处理48h的蚜虫死亡率为62.22%,96h蚜虫死亡率为91.1%,这种纯天然有机物结合高压静电喷雾杀虫方法的发现,为蚜虫的无公害防治提供了一条新的途径.     

Estimation of genetic variance components and heritabilities for cut-flower yield in gerbera using least squares and maximum likelihood methods

Summary Genetic variance components and heritability were estimated for cut-flower yield of gerbera in the Davis population using ordinary least squares and maximum likelihood methods. The overall estimate of narrow-sense heritability is 0.33 based on least squares (LS) and 0.31 based on maximum likelihood (ML). The results of the study indicate that (1) ML and LS provide very similar results if sample size is large enough, suggesting both are useful for plant breeding programs; (2) about one third of the variation in gerbera cut-flower yield is additive, implying selection in cut-flower will be successful; and (3) although additive variation gradually decreased, heritability remained near 0.27 suggesting there is still potential variation in the population for further selection.